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The manuscript describes surgical technique of rumen cannulation in cattle using flexible cannulas, which are necessary in prediction of experimental feeds' potential degradability using an in situ method. The main principles of pre-operative preparation of the experimental animals and their post-operative treatment to ensure their long-term use in experiments with no complications are also discussed. In addition, we provide a brief overview of the history of surgical techniques and procedures used to determine feed digestibility. This overview proves that, despite the efforts to avoid surgeries on experimental animals, these remain actual and necessary.

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