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In this study, the effects of nicotinamide adenine dinucleotide phosphate (NADPH) and phenylalanine on goat sperm motility were investigated. Fresh Shami goat semen was collected and incubated at 37 °C for 45 minutes in Tris-based medium with 1, 2 and 4 mM or without both NADPH and phenylalanine. Sperm motility was assessed using computer assisted sperm analyzer (CASA). Higher velocity values were achieved when 1 and 2 mM of NADPH were added, while the addition of phenylalanine at the same concentrations had reduced the values of all motility parameters, with no significant differences (P > 0.05) compared to the control. Addition of the two substrates at a concentration of 4 mM had a clear negative effect (P < 0.05) on the values of sperm motility parameters as well as on the percentages of spermatozoa subpopulations, especially for the rapid and the static categories. We concluded that both NADPH and phenylalanine have pronounced effects on goat sperm motility and these effects were dependent on concentration. The supplementation of semen media with NADPH at an appropriate concentration can be useful as a stimulating additive for goat sperm motility.
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