Main Article Content

Andrea Svoradová
Marian Machac
Andrej Baláži
Jaromir Vasicek
Rastislav Jurcik
Jan Huba
Peter Chrenek


Viability and sperm motion parameters are the most common indicators of sperm quality. Semen samples (n = 15) of Improved Wallachian (IW) sheep males with no evidence of genital tract infections were used to determine the quality of semen. We applied computer-assisted semen analysis (CASA) system to determine sperm motility parameters. Flow cytometry (FCM) was used to evaluate occurrence of live or apoptotic sperm. Our results showed quite good motility parameters: 81.12 ± 2.72 % of total motility (TM) and 77.91 ± 3.15 % of progressive motility (PM). Moreover, the ratio of viable sperm was 70.77 ± 4.81 % and of apoptotic sperm ˗ 9.37 ± 3.37 %. These results suggest that semen can be collected from IW sheep and used for artificial insemination or cryopreservation for gene bank of animal genetic resources. However, evaluation of higher sample numbers using different methods are needed to analyse the semen quality in details.

Article Details



Abdel-Rahman, H. A., Belely, M. S., Al-Qarawi, A. A. & El-Mougy, S. A. (2000). The relationship between semen quality and mineral composition of semen in various ram breed. Small Ruminant Research, 38(1), 45–49. Doi: 10.1016/s0921-4488(00)00137-1

Aitken, R. J. & Baker, M. A. (2013). Causes and consequences of apoptosis in spermatozoa; contributions to infertility and impacts on development. The International Journal of Developmental Biology, 57(2-4), 265–272. Doi: 10.1387/ijdb.130146ja

Al-Samarrae, S. H. (2009). Semen quality of Arrabi and Karradi Iraqi rams. Diyala Journal of Agricultural Sciences, 1(2), 30–36.

Azizun Nesa, Zohara, B. F., Bari, F. Y. & Alam, M. G. S. (2014). Baseline study of reproductive performances of indigenous rams in Bangladesh. IOSR Journal of Agriculture and Veterinary Sciences, 7(6), 83–89. Doi:10.9790/2380-07618389

Boshoff, N. H., Lambrechts, H., Maree, L., Cloete, S. W. P. & Van der Horst, G. (2018). A novel flush technique to simulate natural dispersal of spermatozoa in the female reproductive tract and expedite motility assessment of fresh ejaculated Merino (Ovis aries) sperm. South African Journal of Animal Science, 48(3), 469–476.

Bucak, M. N., Bodu, M., Başpınar, N., Güngör, Ş., İli, P., Acibaeva, B., Topraggaleh, T. R. & Dursun, Ş. (2019). Influence of ellagic acid and ebselen on sperm and oxidative stress parameters during liquid preservation of ram semen. Cell Journal, 21(1), 7–13. Doi: 10.22074/cellj.2019.5593

Buša, D., Macháč, M., Baláži, A., Svoradová, A., Vašíček, J., Jurčík, R. & Chrenek, P. (2019). Sperm motility of rams from two Slovak sheep breeds. Slovak Journal of Animal Science, 52(4), 178–180.

Chrenek, P., Makarevich, A. V., Kubovicova, E., Bulla, J. & Supuka, P. (2019). Slovak National Animal Breeds. Nitra: Slovak University of Agriculture, 16–20. ISBN 978-80-552-1982-0.

Colas, G. (1981). Seasonal variations of sperm quality in adult Ile-de-France rams. II. Fertilization ability and its relation to qualitative criteria in vitro. Reproduction Nutrition Development, 21(3), 399–407.

David, I., Robert-Granié, C., Manfredi, E., Lagriffoul, G. & Bodin, L. (2008). Environmental and genetic variation factors of artificial insemination success in French dairy sheep. Animal, 2(7), 979–986.

Farah, O. I., Cuiling, L., Jiaojiao, W. & Huiping, Z. (2013). Use of fluorescent dyes for readily recognizing sperm damage. Journal of Reproduction & Infertility, 14(3), 120–125.

Fernandez, M., Giráldez, F. J., Frutos, P., Lavín, P. & Mantecón, A. R. (2004). Effect of undegradable protein supply on testicular size, spermiogram parameters and sexual behaviour of mature Assaf rams. Theriogenology, 62(1-2), 299–310.

Fonseca, J. F., Torres, C. A. A., Maffili, V. V., Borges, A. M., Santos, A. D. F., Rodrigues, M. T. & Oliveira, R. F. M. (2005). The hypo-osmotic swelling test in fresh goat spermatozoa. Animal Reproduction, 2(2), 139-144.

Garner, D. L. & Hafez, E. S. E. (1982). Spermatozoa and seminal plasma. Reproduction in farm animals, 7th ed. Philadelphia, 12–17.

Garner, D. L. & Johnson, L. A. (1995). Viability assessment of mammalian sperm using SYBR-14 and propidium iodide. Biology of Reproduction, 53(2), 276–284.

Gündoğan, M., Yeni, D., Uçar, M. & E. Özenç, E. (2004). Relationship between some reproductive parameters and biochemical properties of blood serum in ram. Archives of Andrology, 50(6), 387–390.

Khalifa, T., Lymberopoulos, A. & Theodosiadou, E. (2013). Association of soybean-based extenders with field fertility of stored ram (Ovis aries) semen: a randomized double-blind parallel group design. Theriogenology, 79(3), 517–527.

Kulíková, B., Baláži, A., Tóthová, J., Jurčík, R., Huba, J. & Chrenek, P. (2018). Dilution factor affects the ability of ram sperm to survive cryopreservation. Slovak Journal of Animal Science, 51(1), 41–44.

Luna-Orozco, J. R., González-Ramos, M. A., Calderón-Leyva, G., Gaytán-Alemán, L. R., Arellano-Rodríguez, F., Ángel-García, O. & Véliz-Deras, F. G. (2019). Comparison of different diluents based on liposomes and egg yolk for ram semen cooling and cryopreservation. Iranian Journal of Veterinary Research, 20(2), 126–130.

Malama, E., Bollwein, H., Taitzoglou, I. A., Theodosiou, T., Boscos, C. M. & Kiossis, E. (2013). Chromatin integrity of ram spermatozoa. Relationships to annual fluctuations of scrotal surface temperature and temperaturehumidity index. Theriogenology, 80(5), 533–541.

Pegg, D. E. (2015). Principles of cryopreservation. Methods in Molecular Biology, 1257, 3–19. Doi: 10.1007/978-1-4939-2193-5_1

Rege, J. E., Toel, F., Mukasa-Mugerwa, E., Tembely, S., Amindo, D., Baker, R. L. & Lahlou-Kassi, A. (2000). Reproductive characteristics of Ethiopian highland sheep. II. Genetic parameters of semen characteristics and their relationships with testicular measurements in ram lambs. Small Ruminant Research, 37(3), 173–187.

Špaleková, E., Makarevich, A. V. & Lukáč, N. (2011). Ram sperm motility parameters under the influence of epidermal growth factor. Veterinary Medicine International, Epub 2011:642931. Doi: 10.4061/ 2011/642931

Toe, F., Lahlou-Kassi, A. & Mukasa-Mugerwa, E. (1994). Semen characteristics of Ile-de-France rams of different age and physical condition. Theriogenology, 42(2), 321–326.

Most read articles by the same author(s)

1 2 > >>