THE USE OF HOMA-IR AND QUICKI IN RODENT DIABETIC MODEL: SHORT COMMUNICATION

Main Article Content

Marcela Capcarova
Anna Kalafova
Rudolf Dupak
Peter Kisska
Monika Schneidgenova

Abstract

Regarding diabetes mellitus type 2 (DMT2) in animal research, a non-invasive and less traumatic method, such as the mathematic calculation of indexes expressing the insulin sensitivity and resistance, is required. There are some methods and formulas for calculation and estimation of insulin resistance. The most well-known validated methods are the homeostatic model assessment of insulin resistance (HOMA-IR) and the quantitative insulin sensitivity check index (QUICKI), which are suitable for clinical and research purposes. The goal of this study was to calculate HOMA-IR and QUICKI indexes in an experiment with Zucker diabetic fatty (ZDF) rats fed normal or high-energy diet. Additionally, the correlations between both models were inquired. Animals were divided into three groups: lean untreated control rats (C, n = 10) fed a complete feed mixture for rats and mouse (10 MJ.kg-1), diabetic rats fed the same chow (E1, 10 MJ.kg-1) and diabetic rats fed high energy diet (E2, enriched KKZ-P/M, 20 MJ.kg-1). After overnight fasting, the rats were monitored for blood glucose level by a FreeStyle Optium Neo Glucose and Ketone Monitoring System (Abbott Diabetes Care Ltd., UK) using test strips. An ELISA commercial kit (Biotech, Bratislava, Slovak Republic) was used to measure the serum content of insulin. Values of fasting plasma insulin and serum glucose were used to calculate HOMA-IR and QUICKI indexes. HOMA-IR and QUICKI significantly differed among the groups. Strong negative correlations were found in dependence on the diet. This study indicated that the calculation of HOMA-IR and QUICKI can potentially be an effective tool in determination, evaluation, onset and progress of DMT2.

Article Details

Section
Short Communication

References

ANTUNA-PUENTE, B., FARAJ, M., KARELIS, A. D., GARREL, D., PRUDHOMME, D., RABASA-LHORET, R. & BASTARD, J. P. (2008). HOMA or QUICKI: Is it useful to test the reproducibility of formulas? Diabetes and Metabolism, 34, 294–296.

ANTUNES, L. C., ELKFURY, J. L., JORNADA, M. N., FOLETTO, K. C. & BERTOLUCI, M. C. (2016). Validation of HOMA-IR in a model of insulin-resistance induced by a high-fat diet in Wistar rats. Archives of Endocrinology and Metabolism, 60, 2016, 138–142.

APPLETON, D. J., RAND, J. S. & SUNVOLD, G. D. (2002). Plasma leptin concentrations are independently associated with insulin sensitivity in lean and overweight cats. Journal of Feline Medicine and Surgery, 4(2), 83–93.

BODEN, G. (2001). Pathogenesis of type 2 diabetes. Insulin resistance. Endocrinology and Metabolism Clinics of North America, 30, 801–815.

BRAY, G. A. (2004). Medical consequences of obesity. Journal of Clinical Endocrinology and Metabolism, 89, 2583–2589.

CAPCAROVA, M., KALAFOVA, A., SCHWARZOVA, M., SOLTESOVA PRNOVA, M., SVIK, K., SCHNEIDGENOVA, M., SLOVAK, L., BOVDISOVA, I., TOMAN, R., LORY, V. & ZORAD, S. (2018a). The high-energy diet affecting development of diabetes symptoms in Zucker diabetic fatty rats. Biologia, 73, 659–671.

CAPCAROVA, M., SOLTESOVA PRNOVA, M., SVIK, K., SCHNEIDGENOVA, M., SLOVAK, L., KISSKA, P., MAMRAKOVA, R., PRISTASOVA, M. & KALAFOVA, A. (2018b). Rodent animal model for research in diabetes: A mini-review. Slovak Journal of Animal Science, 51(3), 138–145.

CHEN, H., SULLIVAN, G., YUE, L. Q., KATZ, A. & QUON, M. J. (2003). QUICKI is a useful index of insulin sensitivity in subjects with hypertension. American Journal of Physiology-Endocrinology and Metabolism, 284, e804–e812.

CHOI, K. & KIM, Y. B. (2010). Molecular mechanism of insulin resistance in obesity and type 2 diabetes. Korean Journal of Internal Medicine, 25(2), 119–129.

GUTCH, M., KUMAR, S., RAZI, S. M., GUPTA, K. & GUPTA, A. (2015). Assessment of insulin sensitivity/resistance. Indian Journal of Endocrinology and Metabolism, 19(1), 160–164.

HAFFNER, S. M., MIETTINEN, H. & STERN, M. P. (1997). The homeostasis model in the San Antonio Heart Study. Diabetes Care, 20(7), 1087–1092.

MARI, A., PACINI, G., MURPHY, E., LUDVIK, B. & NOLAN, J. J. (2001). A model-based method for assessing insulin sensitivity from the oral glucose tolerance test. Diabetes Care, 24, 539–548.

MATHER, K. (2019). Surrogate measures of insulin resistance: of rats, mice, and men. American Journal of Physiology-Endocrinology and Metabolism, 296, e398–e399.

MATTHESW, D. R., HOSKER, J. P., RUDENSKI, A. S., NAYLOR, B. A., TREACHER, D. F. & TURNER, R. C. (1985). Homeostasis model assessment: Insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia, 28, 412–419.

SARAFIDIS, P. A., LASARIDIS, A. N., NILSSON, P. M., STAFILAS, P. C., KANAKI, A., KAZAKOS, K., YOVOS, J. & BAKRIS, G. L. (2007). Validity and reproducibility of HOMA-IR, 1/HOMA-IR, QUICKI and McAuley's indices in patients with hypertension and type II diabetes. Journal of Human Hypertension, 21, 709–716.

WALLACE, T. M., LEVY, J. C. & MATTHEWS, D. R. 2004. Use and abuse of HOMA modelling. Diabetes Care, 27(4), 1487–1495.

YOKOYAMA, H., EMOTO, M., FUJIWARA, S., MOTOYAMA, K., MORIOKA, T., KOMATSU, M., TAHARA, M., SHOJI, T., OKUNO, Y. & NISHIZAWA, Y. (2003). Quantitative insulin sensitivity check index and the reciprocal index of homeostasis model assessment in normal range weight and moderately obese type 2 diabetic patients. Diabetes Care, 26(8), 2426–2432.