USE OF ENTEROCIN M SUBSTANCE APPLIED IN DRINKING WATER AND NATURAL ZEOLITE AS DIETARY SUPPLEMENTS FOR GROWING RABBITS

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Lubica Chrastinova
Maria Chrenkova
Zuzana Formelova
Andrea Laukova
Monika Pogany Simonova
Matus Rajsky
Maria Polacikova
Iveta Placha
Kristina Bacova
Ondrej Bucko
Gabriela Strkolcova
Zuzana Mlynekova
Tomas Rasi
Lubomir Ondruska
Rastislav Jurcik

Abstract

A total number of 72 post-weaned rabbits (aged 35 days, meat line M91 a P91 hybrid rabbits), reared in an intensive breeding in the Slovak Republic, were used in the experiment. The rabbits were randomly divided into 3 similar groups, 24 animals in each group (control -EG1, experimental groups – EG2 and EG3) and kept in standard metal cages, two animals per cage. This study investigated the effects of different feeding on growth performance, chemical composition, fatty acid profile and content of essential amino acids of the Musculus longissimus thoracis and lumborum of growing rabbits. This in vivo study was designed to reveal whether the antimicrobial effect of Enterocin M (produced by non-rabbit origin strain Enteroccocus faecium AL41-CCM8558), is able to influence the meat quality of rabbits. Every day at the same time in the morning the rabbits in EG 2 received 50 μl dose of Ent M per animal per day, administered into their drinking water. The rabbits of experimental group (EG3) had commercial diet enriched with the supplement of 1 % natural zeolite (a product of ZEOCEM Company, Bystré, Slovakia) and the drinking water did not contain any coccidiostatic drugs during the experiment. The experiment was lasted for 42 days. The fat of rabbits in the 2EG had a higher (P ˂ 0.05) concentration of n-3 and n-6, MUFA, while the ratio of SFA in the control was lower (32.92 %) than in 2EG and 3EG reared rabbits (34.61 % vs. 34.71 %). Feeding of natural substances to rabbits did not influence biochemical and zootechnical parameters and it had no negative effect on growth performance in rabbits. It had positive effect on health status and it reduced the number of Eimeria spp. Oocysts were not detected in rabbits' intestinal tract.

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References

AOAC 2005. Official Methods of Analysis. 18th Edition. Association of Official Analytical Chemists, Gaithersburg, USA.

Bannon, C. D., Breen, G. J., Hal, N. & O'Rourke, K. L. (1982). Analysis of fatty acids methylesters with accuracy and reliability. Journal of Chromatography A, 247(1), 71–89.

Bianchi, M., Petracci, M. & Cavani, C. (2006). Effects of dietary inclusion of dehydrated lucerne and whole linseed on rabbit meat quality. World Rabbit Science, 14(4), 247–258.

Chrastinová, Ľ., Chrenková, M., Lauková A., Polačiková, M., Simonová, M., Szabóová, R., Strompfová, V., Ondruška, Ľ., Chlebec, I., Parkányi, V., Rafay, J. & Vasilková, Z. (2010). Influence of selected phytoadditives and probiotics on zootechnical performance, caecal parameters and meet quality of rabbits. Archiva Zootechnica, 13, 30–35.

Chrastinová, Ľ., Lauková, A., Chrenková, M., Poláčiková, M., Formelová, Z., Kandričáková, A., Glatzová., E., Ščerbová, J., Bučko, O., Ondruška, Ľ., Szabóová, R. & Jurčík, R. (2018). Effects of Enterocin M and Durancin ED26E/7 substances applied in drinking water on the selected carcass characteristics and meat quality of broiler rabbits. Archiva Zootechnica, 21(1), 5–17.

Combes, S. (2004). Nutritional value of rabbit meat: a review. Productions Animales, 17(5), 373–383.

Dalle Zotte, A. (2002). Perception of rabbit meat quality and major factors influencing the rabbit carcass and meat quality. Livestock Production Science, 75, 11–32. Doi:10.1016/S0301-6226 (01) 00308-6

Dalle Zotte, A., Szendrӧ, S. (2011). The role of rabbit meat as functional food. Meat Science, 88, 319–331. Doi:10.1016/j.meatsci.2011.02.017

Franz, CH. M. A. P., van Belkum, M. J., Holzapfel, W. H., Abriouel, G. & Gálvéz, A. (2007). Diversity of enterococcal bacteriocins and their grouping in a new classification scheme. FEMS Microbiology Reviews, 31, 293–307.

Gondret, F., Juin, H., Mourot, J. & Bonneau, M. (1998). Effect of age at slaughter on chemical traits and sensory quality of Longissimus lumborum muscle in the rabbit. Meat Science, 48, 181–187. Doi:10.1016/S0309-1740(97) 00088-0

Hašek, A. & Palanská, O. (1976). Determination of water holding capacity in the meat by instruments with constant pressure. Poultry Industry, 18, 228–233. (In Slovak).

Lauková, A., Chrastinová, Ľ., Pogány Simonová, M., Strompfová, V., Plachá, I., Čobanová, K., Formelová, Z., Chrenková, M. & Ondruška, Ľ. (2012). Enterococcus faecium Al41: Its Enterocin M and their beneficial use in rabbit's husbandry. Probiotics and Antimicrobial Proteins, 4, 243–249. https://doi.org/10.1007/s12602-012-9118-7

Lauková, A., Strompfová, V., Plachá, I., Čobanová, K., Faix, Š. & Pogány Simonová, M. (2015). Beneficial effect of enterocin M-producing probiotic strain Enterococcus faecium Al41 in model experiment with hens. Global Journal of Animal Scientific Research, 3(1), 206–213.

Lauková, A., Pogány Simonová, M., Chrastinová, Ľ., Plachá, I., Čobanová, K., Formelová, Z., Chrenková, M., Ondruška, Ľ. & Strompfová, V. (2016). Benefits of combinative application of probiotic, enterocin M-producing strain Enterococcus faecium AL41 and Eleutherococcus senticosus in rabbits. Folia Microbiologica, 61, 169–177.
https://doi.org/10.1007/s12223-015-0423-x

Kalafová, A., Bulla, J., Bučko, O., Emrichová, J., Zbýňovská, K., Petruška, P., Schneidgenová, M., Chrastinová, Ľ., Ondruška, Ľ. Jurčík, R., Čupka, P., Mellen, M. & Capcarová, M. (2014). Meat quality of female rabbits following administration of epicatechin. Maso International, 4(1), 71–75.

Kalafová, A., Hrnčar, C., Zbýňovská, K., Bučko, O., Hanusová, M., Kapustová, Z., Schneidgenová, M., Bielik, P. & Capcarová, M. (2018). The effects of dietary probiotics and humic acid on meat quality of Japanese quail including sex-related differences and economical background. Biologia, 73, 765–771. https://doi:10.2478/s11756-018-0085-7

McMaster. (1986). Modified McMaster Egg counting for quantification of nematode eggs. Ministry of Agriculture, Fisheries and Food, In: Manual of veterinary parasitological laboratory techniques, London, UK.

Ouhayoun, J. (1992). Rabbit meat characteristics and qualitative variability. Cuni-Sciences, 7, 1–15.

Pogány Simonová, M., Chrastinová, L’., Mojto, J., Lauková, A., Szabóová, R. & Rafay, J. (2010). Quality of rabbit meat and phyto-additives. Czech Journal of Food Science, 28, 161–167.

Pogány Simonová, M., Lauková, A., Žitňan, R. & Chrastinová, Ľ. (2015). Effect of rabbit-origin enterocin-producing strain Enterococcus faecium CCM7420 application on growth performance and gut morphometry in rabbits. Czech Journal of Animal Science, 60(11), 509–512.

Rafay, J., Novotná, K., Mojto, J., Bozic, A. & Chrenek, P. (2008). Some meat utility and quality traits of transgenic rabbit. Slovak Journal of Animal Science, 41(3), 121–125.

Shadrin, A. M. (1988). Syberian zeolite in rations of animals and birds. In: "Teoreticheskie i prikladnye problemy vnedrenia prirodnych zeolitov v narodnom choziaystve RSFSR" Kemerovo, p. 68–70.

Shibaev, N. V., Butko, M. P. (1986). Influence of zeolite on production, veterinary-sanitary indices and biological value of young cattle meat. In: Tez. Dokl. konferentsii, "Dobycha, pererabotka i primenenie zeolitov", Tbilisi, p. 149–149.

STN 570185 (1962). Slovak Technical Standards. Examination of meat, meat products and canned meat and prepared meats in cans. Chemical and physical methods. Bratislava, Slovakia.

Strmiska, F., Holčíkova, K., Simonová, E., Mrázova, E., Hodeková, J., Votašaková, A., Pristašová, M., Strmiska, J., Strmisková, G., Kruparová, M., Gola, J., Papajová , H., Mareš, J., Kostkanová, E. & Ehrenhaft, B. (1988). Food components (In Slovak). Potravinové suroviny, Food Tables I. Journal of Food and Nutrition Research, 189 p.

Strompfová, V., Ščerbová, J., Plachá, I., Kubašová, I., Chrastinová, Ľ., Kandričáková, A. & Lauková, A. (2017). Enterocins, beneficial additives in animals. In: Proceedings of the International Scientific Conference on Probiotics and Prebiotics IPC2017, June 20th -22nd, 2017, Budapest, Hungary, p. 39–40. ISBN: 978-80-89589-16-6.

Szabóová, R., Lauková, A., Chrastinová, Ľ., Pogány Simonová, M., Strompfová, V., Plachá, I., Čobanová, K., Vasilková, Z. & Chrenková, M. (2011). Enterocin 4231 produced by Enterococcus faecium CCM 4231 and its use in rabbits. Acta Veterinaria (Beograd), 61 (5-6), 523–529. Doi: 10.2298/AVB 1106523S

Van Soest, J. P., Robertson, J. B. & Lewis, B. A. (1991). Methods for dietary fibre, neutral detergent fibre and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74, 3583–3594.

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