QUALITY EVALUATION OF FRESH GANDER SEMEN OF SLOVAK WHITE GOOSE BY CASA AND FLOW CYTOMETRY

Main Article Content

Andrea Svoradová
Andrej Baláži
Jaromír Vašíček
Cyril Hrnčár
Peter Chrenek

Abstract

In this preliminary study, the quality of fresh gander semen from the original Slovak White goose (SW) breed was analysed. Semen was collected from three (SW, n = 3) ganders into prepared sterile tubes by dorso-abdominal massage. Firstly, volume of individual gander semen was determined. Afterward, the concentration, motility parameters and sperm viability were evaluated using computer assisted semen analyser (CASA) and flow cytometry, respectively.


Our results showed that the volume of individual semen samples varied from 0.05 to 0.38 ml. No significant differences in concentration and total motility of fresh semen was found among the ganders tested. However, significant differences (P ≤ 0.05) in progressive movement of fresh semen between two males (SW2 and SW3) were observed. Moreover, differences (P ≤ 0.05) in the percentage of apoptotic and necrotic sperm between two males (SW1 and SW3) were revealed by a flow cytometry. These preliminary data suggest that the objective assessment of fresh gander sperm motility may be an effective indicator of frozen-thawed semen quality. Therefore, regular semen assessment is required in order to preserve good-quality insemination doses from native breeds.

Article Details

Section
Short Communication

References

Bakst, M. R., Wishart, G. & Brillard, J. P. 1994. Oviductal spermatozoa selection, transport, and storage in poultry. Poultry Science Review, 5, 17–143.

Christensen, P., Hansen, C., Liboriussen, T. & Lehn-Jensen, H. 2005. Implementation of flow cytometry for quality control in four danish bull studs. Animal Reproduction Science, 85, 201–208.

Christensen, P., Stenvang, J. P. & Godfrey, W. 2004. A flow cytometric method for rapid determination of sperm concentration and viability in mammalian and avian semen. Journal of Andrology, 25(2), 255–264.

European Commission. Legislation for the protection of animals used for scientific purposes. Horizontal legislation on the protection of animals used for scientific purpose. 2016. Available at: http://http://ec.europa.eu/environment/chemicals/lab_animals/
legislation_en.htm


Froman, D. P. & Feltmann, A. J. 2000. Sperm mobility: Phenotype in roosters (Gallus domesticus) determined by concentration of motile sperm and straight line velocity. Biology of Reproduction, 62, 303–309.

Froman, D. P. & Feltmann, A. J. 1998. Sperm mobility: A quantitative trait of the domestic fowl (Gallus domesticus). Biology of Reproduction, 58, 379–384.

Froman, D. P., Bowling, E. R. & Wilson, J. L. 2003. Sperm mobility phenotype not determined by sperm quality index. Poultry Science, 82, 496–502.

Gee, G. F. & Sexton, T. J. 1990. Cryogenic preservation of semen from the Aleutian Canada goose. Zoo Biology, 9, 361–71.

Idziorek, T., Estaquier, J., De Bels, F. & Ameisen, J. C. 1995. YOPRO-1 permits cytofluorometric analysis of programmed cell death (apoptosis) without interfering with cell viability. Journal of Immunological Methods, 2, 249–258.

Łukaszewicz, E. 2002. An effective method for freezing White Italian gander semen. Theriogenology, 58, 19–27.

Łukaszewicz, E., Chrzanowska, M., Jerysz, A. & Chełmonska, B. 2004. Attempts on freezing the Greylag (Anser anser L.) gander semen. Animal Reproduction Science, 80, 163–173.

Łukaszewicz, E., Kruszyński, W. & Fujihara, N. 2003. Effect of age on quality of fresh and frozen-thawed semen in White Italian ganders. Asian Journal of Andrology, 5, 89–93.

Martin, G., Sabido, O., Durand, P. & Levy, R. 2004. Cryopreservation induces an apoptosis like mechanism in bull sperm. Biology of Reproduction, 71, 28–37.

Martínez-Pastor, F., Mata-Campuzano, M., Alvarez-Rodriguez, M., Alvarez, M., Anel, L. & De Paz, P. 2010. Probes and techniques for sperm evaluation by flow cytometry. Reproduction in Domestic Animals, 45, 67–78.

Roth, B. L., Poot, M., Yue, S. T. & Millard, P. J. 1997. Bacterial viability and antibiotic susceptibility testing with SYTOX green nucleic acid stain. Applied and Environmental Microbiology, 63, 2421–2431.

Tai, J. J. L., Chen, J. C., Wu, K. C., Wang, S. D. & Tai, C. 2001. Cryopreservation of gander semen. British Poultry Science, 42, 384–388.

Waberski, D., Schapmann, E., Henning, H. & Riesenbeck, A., Brandt, H. 2011. Sperm chromatin structural integrity in normospermic boars is not related to semen storage and fertility after routine AI. Theriogenology, 75, 337–345.

Rijsselaere, T., Van Soom, A., Tanghe, S., Coryn, M., Maes, D. & De Kruif, A. 2005. New techniques for the assessment of canine semen quality: a review. Theriogenology, 64(3), 706–719.

Most read articles by the same author(s)