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The aim of this study was to examine possible effect of amygdalin substance applied to rabbit by different ways on the occurrence of aneuploidy. Totally 30 adult females of the New Zealand White line were used in the experiment. Animals were divided into four experimental groups and one control group, each consisted of five individuals. In groups P1 and P2, isolated amygdalin substance was administered intramuscularly (P1: 0.6 mg.kg-1; P2: 3 mg.kg-1 of body weight), whilst in P3 and P4 the animals were fed by a mixture of commercial diet and apricot seeds (P3: 60 mg.kg-1; P4: 300 mg.kg-1 of body weight). Experimental treatment lasted one month of continuous amygdalin application (one experimental dose applied per day in each group). Aneuploidy assay was performed on peripheral blood lymphocytes arrested at the metaphase stage by colcemid solution (0.6μg.ml-1). The average occurrence of diploidy versus aneuploidy (presented by hypodiploid nuclei) was detected as follows: P1- 76 % vs 21.6 %; P2 - 72.8 % vs 27.2 %; P3 - 82.4 % vs 17.6 %; P4 - 70.4 % vs 29.6 %; Control - 70.4 % vs 26.8 %. Low incidence of polyploid cells was found in P1 (2.4 %) and control (2.8 %) groups. In conclusion, no significant effect of amygdalin on aneuploidy occurrence in rabbit blood cells was found.